RUNX2 Recombinant antibody

RUNX2 Uni-rAb^TM Recombinant Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

1G5

Reviews (2)

Cat no : 82636-2-RR

Synonyms

AML3, CBF alpha 1, CBFA1, CCD, CCD1, Oncogene AML 3, OSF 2, OSF2, PEA2 alpha A, PEA2aA, PEBP2 alpha A, PEBP2A, PEBP2A1, PEBP2A2, PEBP2aA, PEBP2aA1, RUNX2

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Validation Data Gallery

• 

  WB analysis using 82636-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82636-2-RR (RUNX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of ROS1728 using 82636-2-RR

  IP result of anti-RUNX2 (IP:82636-2-RR, 4ug; Detection:82636-2-RR 1:500) with ROS1728 cells lysate 2080 ug.

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Tested Applications

• Positive WB detected in: PC-3 cells, Saos-2 cells, C2C12 cells
• Positive IP detected in: ROS1728 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

82636-2-RR targets RUNX2 in WB, IP, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: runt-related transcription factor 2
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 57-60 kDa
• GenBank Accession Number: NM_004348
• Gene Symbol: RUNX2
• Gene ID (NCBI): 860
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RUNX2, also named as AML3, CBFA1, OSF2 and PEBP2A, is a Transcription factor which involved in osteoblastic differentiation and skeletal morphogenesis. It is essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters By similarity. RUNX2 inhibits MYST4-dependent transcriptional activation.

Publications

Species	Application	Title
mouse	WB	Intractable Rare Dis Res; Role of IFITM2 in osteogenic differentiation of C3H10T1/2 mesenchymal stem cells; Authors - Yongtao Zhang
human	WB	Bone; CTSD upregulation as a key driver of spinal ligament abnormalities in spinal stenosis; Authors - Lei Li
human	WB	Arch Oral Biol; Diagnostic significance of LncRNA MIAT in periodontitis and the molecular mechanisms influencing periodontal ligament fibroblasts via the miR-204-5p/DKK1 axis; Authors - Yu Ren
human	WB	Life Sci; Circ_0114581 promotes osteogenic differentiation of BMSCs via the MiR-155-5p/HNRNPA3 axis; Authors - Hao Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Charlotte (Verified Customer) (07-22-2024)

I used this antibody in WB in cells that differentiate into osteoblast lineage upon stimulation. I had a strong visible band at the expected size

• Applications: Western Blot
• Primary Antibody Dilution: 1/2000
• Cell Tissue Type: C3H10T1/2

RUNX2 Antibody Western Blot validation (1/2000 dilution) in C3H10T1/2 (Cat no:82636-2-RR)

FH

Udesh (Verified Customer) (11-26-2023)

Worked well for ADSCs at 1:1000

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: ADSCs