RUNX1T1 Monoclonal antibody, PBS Only

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  WB analysis of HEK-293 using 67086-1-Ig (same clone as 67086-1-PBS)

  WB result of RUNX1T1 antibody (67086-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RUNX1T1 transfected HEK-293 cells. This data was developed using the same antibody clone with 67086-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67086-1-Ig (same clone as 67086-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67086-1-Ig (RUNX1T1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67086-1-PBS in a different storage buffer formulation.

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  WB analysis using 67086-1-Ig (same clone as 67086-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67086-1-Ig (RUNX1T1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67086-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 67086-1-Ig (same clone as 67086-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67086-1-Ig (RUNX1T1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67086-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 67086-1-Ig (same clone as 67086-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67086-1-Ig (RUNX1T1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67086-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 67086-1-Ig (same clone as 67086-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67086-1-Ig (RUNX1T1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67086-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 67086-1-Ig (same clone as 67086-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67086-1-Ig (RUNX1T1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67086-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67086-1-Ig (same clone as 67086-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67086-1-Ig (RUNX1T1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67086-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67086-1-Ig (same clone as 67086-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67086-1-Ig (RUNX1T1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67086-1-PBS in a different storage buffer formulation.

RUNX1T1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 67086-1-PBS

Clone No.2G2F7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, Indirect ELISA

Synonyms

Eight twenty one protein, Cyclin-D-related protein, CDR, CBFA2T1, AML1T1

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Product Information

67086-1-PBS targets RUNX1T1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RUNX1T1 fusion protein Ag7893
• Full Name: runt-related transcription factor 1; translocated to, 1 (cyclin D-related)
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC005850
• Gene Symbol: RUNX1T1
• Gene ID (NCBI): 862
• RRID: AB_2882394
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q06455
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

RUNX1T1 is a putative zinc finger transcription factor and oncoprotein. In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 gene fused to the 3'-region of this gene. Various transcript of the fusion gene has been reported. RUNX1T1 exists some isoforms with MV 68, 67,64, 48 and 44 kDa. The calcualted molecular weight of RUNX1T1 is 67 kDa, but modified RUNX1T1is about 70-75 kDa.