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WB analysis of mouse lung using 19180-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 19180-1-AP (RUFY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 19180-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19180-1-AP (RUFY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 19180-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19180-1-AP (RUFY2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse ovary using 19180-1-AP
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 19180-1-AP (RUFY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 19180-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 19180-1-AP (RUFY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 19180-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 19180-1-AP (RUFY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human testis using 19180-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 19180-1-AP (RUFY2 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 19180-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 19180-1-AP (RUFY2 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human brain using 19180-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 19180-1-AP (RUFY2 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human brain using 19180-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 19180-1-AP (RUFY2 antibody) at dilution of 1:100 (under 40x lens).

RUFY2 Polyclonal antibody

Name: RUFY2 Polyclonal antibody
Brand: Proteintech
SKU: 19180-1-AP
Rating: 5.00 (1 reviews)

RUFY2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 19180-1-AP

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ZFYVE13, RUN and FYVE domain-containing protein 2, RABIP4R, Rab4-interacting protein related, KIAA1537

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	PC-3 cells, mouse lung tissue, mouse ovary tissue, SH-SY5Y cells, U-251 cells, rat lung tissue
Positive IHC detected in	human testis tissue, human brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19180-1-AP targets RUFY2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RUFY2 fusion protein Ag5938 Predict reactive species
Full Name	RUN and FYVE domain containing 2
Calculated Molecular Weight	403 aa, 46 kDa
Observed Molecular Weight	65-70 kDa
GenBank Accession Number	BC041092
Gene Symbol	RUFY2
Gene ID (NCBI)	55680
RRID	AB_10643382
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8WXA3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.