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RUFY1 Polyclonal antibody, PBS Only

RUFY1 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13498-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RUFY1 Polyclonal antibody (13498-1-AP)

    WB analysis of HeLa using 13498-1-AP (same clone as 13498-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 13498-1-AP (RUFY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13498-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using RUFY1 Polyclonal antibody (13498-1-AP)

    IP experiment of HeLa cells using 13498-1-AP (same clone as 13498-1-PBS)

    IP result of anti-RUFY1 (13498-1-AP for IP and Detection). This data was developed using the same antibody clone with 13498-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RUFY1 Polyclonal antibody (13498-1-AP)

    IF Staining of HeLa using 13498-1-AP (same clone as 13498-1-PBS)

    Immunofluorescent analysis of Hela cells, using RUFY1 antibody 13498-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 13498-1-PBS in a different storage buffer formulation.

Product Information

13498-1-PBS targets RUFY1 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RUFY1 fusion protein Ag4324
Full Name RUN and FYVE domain containing 1
Calculated Molecular Weight 708 aa, 80 kDa
Observed Molecular Weight 69 kDa, 80 kDa
GenBank Accession NumberBC032571
Gene Symbol RUFY1
Gene ID (NCBI) 80230
RRIDAB_2183747
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Rab proteins form the largest group within the Ras superfamily of small GTPases, which acts as molecular switches by cycling between their inactive active GTP-bound and GDP-bound forms [PMID:15520808]. The small GTP-binding protein Rab4 has been involved in the recycling of αvβ3 integrins in response to platelet-derived growth factor (PDGF) stimulation suggesting a role for Rab4 in cell adhesion and migration [PMID:17001082]. Rabip4 (Rab4 interacting protein), which possesses a RUN domain, two coiled-coil domains and a FYVE finger, was identified as an effector of Rab4, a small GTPase that plays a role in the endocytotic pathway in all cell types [PMID:11172003]. RUFY1 exsits three isoforms and molecular weight of isoforms are 80 kDa,69 kDa and 45 kDa.