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RUFY1 Polyclonal antibody, PBS Only

RUFY1 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13498-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RUFY1 Polyclonal antibody (13498-1-AP)

    WB analysis of HeLa using 13498-1-AP (same clone as 13498-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 13498-1-AP (RUFY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13498-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using RUFY1 Polyclonal antibody (13498-1-AP)

    IP experiment of HeLa cells using 13498-1-AP (same clone as 13498-1-PBS)

    IP result of anti-RUFY1 (13498-1-AP for IP and Detection). This data was developed using the same antibody clone with 13498-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RUFY1 Polyclonal antibody (13498-1-AP)

    IF Staining of HeLa using 13498-1-AP (same clone as 13498-1-PBS)

    Immunofluorescent analysis of Hela cells, using RUFY1 antibody 13498-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 13498-1-PBS in a different storage buffer formulation.

Product Information

13498-1-PBS targets RUFY1 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RUFY1 fusion protein Ag4324
Full Name RUN and FYVE domain containing 1
Calculated Molecular Weight 708 aa, 80 kDa
Observed Molecular Weight 69 kDa, 80 kDa
GenBank Accession NumberBC032571
Gene Symbol RUFY1
Gene ID (NCBI) 80230
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Rab proteins form the largest group within the Ras superfamily of small GTPases, which acts as molecular switches by cycling between their inactive active GTP-bound and GDP-bound forms [PMID:15520808]. The small GTP-binding protein Rab4 has been involved in the recycling of αvβ3 integrins in response to platelet-derived growth factor (PDGF) stimulation suggesting a role for Rab4 in cell adhesion and migration [PMID:17001082]. Rabip4 (Rab4 interacting protein), which possesses a RUN domain, two coiled-coil domains and a FYVE finger, was identified as an effector of Rab4, a small GTPase that plays a role in the endocytotic pathway in all cell types [PMID:11172003]. RUFY1 exsits three isoforms and molecular weight of isoforms are 80 kDa,69 kDa and 45 kDa.