RTN4IP1 Polyclonal antibody

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  WB analysis using 15636-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15636-1-AP (RTN4IP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 15636-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RTN4IP1 antibody (15636-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

• 

  IF Staining of HepG2 using 15636-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RTN4IP1 antibody (15636-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

RTN4IP1 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 15636-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

NIMP, NOGO-interacting mitochondrial protein, Reticulon-4-interacting protein 1, mitochondrial

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, Jurkat cells, MCF-7 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15636-1-AP targets RTN4IP1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RTN4IP1 fusion protein Ag8100
• Full Name: reticulon 4 interacting protein 1
• Calculated Molecular Weight: 396 aa, 44 kDa
• Observed Molecular Weight: 32-44 kDa, 24 kDa
• GenBank Accession Number: BC006399
• Gene Symbol: RTN4IP1
• Gene ID (NCBI): 84816
• RRID: AB_3669217
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8WWV3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RTN4IP1 (Reticulon-4-interacting protein 1, mitochondrial), also known as NIMP. It colocalizes with the endoplasmic reticulum HSPA5 at spots corresponding to contacts with mitochondria (PMID: 26593267), which is widely expressed in mitochondria-enriched tissues. Found in heart, muscle, kidney, liver, brain and placenta (PMID: 12067236). The protein plays a role in the regulation of retinal ganglion cell (RGC) neurite outgrowth, and hence in the development of the inner retina and optic nerve. Appears to be a potent inhibitor of regeneration following spinal cord injury. The protein has three isoforms with molecular weights of 43 kDa, 32 kDa and 24 kDa respectively.