RTN1 (Isoform RTN1-C) Polyclonal antibody

• Featured Product
• KD/KO Validated

RTN1 (Isoform RTN1-C) Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 15048-1-AP

Synonyms

NSP, NSP-C, reticulon 1, RTN1, RTN1 (Isoform RTN1-C), RTN1-C

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Validation Data Gallery

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  WB analysis using 15048-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15048-1-AP (RTN1 (Isoform RTN1-C) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15048-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15048-1-AP (RTN1 (Isoform RTN1-C) antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15048-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15048-1-AP (RTN1 (Isoform RTN1-C) antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse cerebellum using 15048-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15048-1-AP (RTN1 (Isoform RTN1-C) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 15048-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15048-1-AP (RTN1 (Isoform RTN1-C) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15048-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15048-1-AP (RTN1 (Isoform RTN1-C) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15048-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15048-1-AP (RTN1 (Isoform RTN1-C) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 15048-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15048-1-AP (RTN1 (Isoform RTN1-C) antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 15048-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15048-1-AP (RTN1 (Isoform RTN1-C) antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 15048-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15048-1-AP (RTN1 (Isoform RTN1-C) antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 15048-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15048-1-AP (RTN1 (Isoform RTN1-C) antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, rat brain tissue
• Positive IHC detected in: mouse cerebellum tissue, human brain tissue, human placenta tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IF: See 1 publications below

Product Information

15048-1-AP targets RTN1 (Isoform RTN1-C) in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RTN1 (Isoform RTN1-C) fusion protein Ag7031
• Full Name: reticulon 1
• Calculated Molecular Weight: 84 kDa, 39 kDa, 24 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC000314
• Gene Symbol: RTN1-C
• Gene ID (NCBI): 6252
• RRID: AB_2185981
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER) (PMID: 18177508). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). They are involved in shaping the tubular endoplasmic reticulum network, membrane trafficking, inhibition of axonal growth, and apoptosis (PMID: 24218324). Four mammalian reticulons (RTN1-4) exist. Human RTN1 (also known as NSP) gene is expressed predominantly in neuroendocrine tissues, and produces three isoforms termed RTN1-A (NSP-A), RTN1-B (NSP-B), and RTN1-C (NSP-C). This antibody is raised against the full-length protein of human RTN1-C.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Combinatorial selective ER-phagy remodels the ER during neurogenesis; Authors - Melissa J Hoyer
mouse, rat	WB, IF	Cell Death Dis; RTN1-C mediates cerebral ischemia/reperfusion injury via ER stress and mitochondria-associated apoptosis pathways.; Authors - Lingli Gong ; KD Validated
mouse	WB	Hum Mol Genet; Transverse endoplasmic reticulum expansion in hereditary spastic paraplegia corticospinal axons.; Authors - Peng-Peng Zhu
rat,mouse	WB	Acta Biochim Biophys Sin (Shanghai); RTN1-C mediates cerebral ischemia/reperfusion injury via modulating autophagy.; Authors - Jun Ling ; KD Validated
mouse	WB	NPJ Parkinsons Dis; Deficiency of Perry syndrome-associated p150Glued in midbrain dopaminergic neurons leads to progressive neurodegeneration and endoplasmic reticulum abnormalities; Authors - Jia Yu
mouse	WB	Brain Res Bull; RTN1-C is involved in high glucose-aggravated neuronal cell subjected to oxygen-glucose deprivation and reoxygenation injury via endoplasmic reticulum stress.; Authors - Muya Lin