Tested Applications
| Positive WB detected in | mouse testis tissue, NIH/3T3 cells, rat testis tissue |
| Positive IHC detected in | rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
| IF | See 6 publications below |
Product Information
23253-1-AP targets RSPH9 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RSPH9 fusion protein Ag19745 Predict reactive species |
| Full Name | radial spoke head 9 homolog (Chlamydomonas) |
| Calculated Molecular Weight | 276 aa, 31 kDa |
| Observed Molecular Weight | 31-35 kDa |
| GenBank Accession Number | BC029519 |
| Gene Symbol | RSPH9 |
| Gene ID (NCBI) | 221421 |
| RRID | AB_2879240 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q9H1X1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RSPH9 (Radial Spoke Head 9 Homolog) is a protein involved in the structure and function of cilia and flagella. Cilia and flagella are hair-like structures that extend from the surface of many eukaryotic cells and play critical roles in cell motility, sensory perception, and signaling. The RSPH9 protein is a component of the radial spoke, a structure within the axoneme (the core of cilia and flagella) that is essential for the regulation of ciliary and flagellar movement.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RSPH9 antibody 23253-1-AP | Download protocol |
| IHC protocol for RSPH9 antibody 23253-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Elife LRRC23 truncation impairs radial spoke 3 head assembly and sperm motility underlying male infertility | ||
Hum Reprod CALR3 defects disrupt sperm-zona pellucida binding in humans: new insights into male factor fertilization failure and relevant clinical therapeutic approaches | ||
Hum Mol Genet Loss of DRC1 function leads to multiple morphological abnormalities of the sperm flagella and male infertility in human and mouse. | ||
Eur J Hum Genet Bi-allelic variants in human TCTE1/DRC5 cause asthenospermia and male infertility. | ||
Cell Rep Differential requirements of IQUB for the assembly of radial spoke 1 and the motility of mouse cilia and flagella | ||
EBioMedicine CFAP70 is a solid and valuable target for the genetic diagnosis of oligo-astheno-teratozoospermia in infertile men |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Célia (Verified Customer) (10-28-2024) | I tested 1/100 to be sure that it works in IF but the concentration was a little high so 1/200 should still be fine.
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