RSL24D1 Polyclonal antibody

RSL24D1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 25190-1-AP

Synonyms

C15orf15, HRP L30 iso, L30, Ribosomal protein L24 like, RLP24, RPL24, RPL24L, RSL24D1, TVAS3

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Validation Data Gallery

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  WB analysis of HepG2 using 25190-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25190-1-AP (RSL24D1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 25190-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25190-1-AP (RSL24D1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 25190-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 25190-1-AP (RSL24D1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 25190-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25190-1-AP (RSL24D1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 25190-1-AP

  Immunofluorescent analysis of HepG2 cells using 25190-1-AP (RSL24D1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: HepG2 cells, HL-60 cells, PC-3 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 2 publications below

Product Information

The immunogen of 25190-1-AP is RSL24D1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RSL24D1 fusion protein Ag18195
• Full Name: ribosomal L24 domain containing 1
• Calculated Molecular Weight: 163 aa, 20 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC008409
• Gene Symbol: RSL24D1
• Gene ID (NCBI): 51187
• RRID: AB_2879950
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	Cell Rep; Labeling of heterochronic ribosomes reveals C1ORF109 and SPATA5 control a late step in human ribosome assembly.; Authors - Chunyang Ni
human	WB	RNA; Human pre-60S assembly factors link rRNA transcription to pre-rRNA processing; Authors - Mason A McCool
human	IF	mBio; Genome-Scale CRISPR Screening Reveals Host Factors Required for Ribosome Formation and Viral Replication; Authors - Maikke B Ohlson
human	WB	Cell; The SPATA5-SPATA5L1 ATPase complex directs replisome proteostasis to ensure genome integrity; Authors - Vidhya Krishnamoorthy