RRN3 Polyclonal antibody

RRN3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, chIP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 25918-1-AP

Synonyms

RRN3, TIF IA, TIFIA

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Validation Data Gallery

• 

  WB analysis of HeLa using 25918-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25918-1-AP (RRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 25918-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25918-1-AP (RRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 25918-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25918-1-AP (RRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 25918-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25918-1-AP (RRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 25918-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25918-1-AP (RRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 25918-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25918-1-AP (RRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 25918-1-AP

  IP result of anti-RRN3 (IP:25918-1-AP, 4ug; Detection:25918-1-AP 1:500) with HeLa cells lysate 3200ug.

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, K-562 cells, mouse testis tissue
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• ChIP: See 1 publications below

Product Information

The immunogen of 25918-1-AP is RRN3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RRN3 fusion protein Ag23207
• Full Name: RRN3 RNA polymerase I transcription factor homolog (S. cerevisiae)
• Observed Molecular Weight: 74 kDa
• GenBank Accession Number: BC132688
• Gene Symbol: RRN3
• Gene ID (NCBI): 54700
• RRID: AB_2880296
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	chIP	Biochem Biophys Res Commun; Nucleolar localization signal and histone methylation reader function is required for SPIN1 to promote rRNA gene expression.; Authors - Xiaolei Zhang
	WB	Mol Cell; Control of ribosomal RNA synthesis by hematopoietic transcription factors; Authors - Charles Antony