RRM2B,p53R2 Polyclonal antibody

• Featured Product
• KD/KO Validated

RRM2B,p53R2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 18005-1-AP

Synonyms

p53R2, RRM2B, RRM2B,p53R2

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Validation Data Gallery

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  WB analysis using 18005-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18005-1-AP (RRM2B,p53R2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 18005-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18005-1-AP (RRM2B,p53R2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 18005-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18005-1-AP (RRM2B,p53R2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 18005-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 18005-1-AP (RRM2B,p53R2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18005-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18005-1-AP (RRM2B,p53R2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A431 cells, PC-3 cells, MDA-MB-453s cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

18005-1-AP targets RRM2B,p53R2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RRM2B,p53R2 fusion protein Ag12499
• Full Name: ribonucleotide reductase M2 B (TP53 inducible)
• Calculated Molecular Weight: 351 aa, 41 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC108261
• Gene Symbol: RRM2B
• Gene ID (NCBI): 50484
• RRID: AB_2878480
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Ther; DGCR8/ZFAT-AS1 Promotes CDX2 Transcription in a PRC2 Complex-Dependent Manner to Facilitate the Malignant Biological Behavior of Glioma Cells.; Authors - Fangfang Zhang ; KD Validated