RRM2 Monoclonal antibody

RRM2 Monoclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

2A9A7

Cat no : 67006-1-Ig

Synonyms

R2, RR2, RR2M, RRM2



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, A431 cells, Jurkat cells, K-562 cells, THP-1 cells
Positive IP detected inHEK-293 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67006-1-Ig targets RRM2 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RRM2 fusion protein Ag28664
Full Name ribonucleotide reductase M2 polypeptide
Calculated Molecular Weight 389 aa, 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC030154
Gene Symbol RRM2
Gene ID (NCBI) 6241
RRIDAB_2882323
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribonucleotide reductase M2 subunit is one of two subunits that constitute ribonucleotide reductase, the enzyme that catalyzes the conversion of ribonucleotide 5'-diphosphates into 2'-deoxyribonucleotides, a rate-limiting step in the production of 2'-deoxyribonucleoside 5'-diphosphates (dNTP) required for DNA synthesis and repair that is required for DNA synthesis and repair [PMID:20825972, 19250552]. RRM2 is only expressed during the late G1/early S phase, and degraded in late S phase, and the activity of RNR, and therefore DNA synthesis and cell proliferation, is controlled during the cell cycle by the synthesis and degradation of RRM2 subunit [PMID:3894352].

Protocols

Product Specific Protocols
WB protocol for RRM2 antibody 67006-1-IgDownload protocol
IF protocol for RRM2 antibody 67006-1-IgDownload protocol
IP protocol for RRM2 antibody 67006-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Neoplasma

The SNHG16/miR-30a axis promotes breast cancer cell proliferation and invasion by regulating RRM2.

Authors - S M Du
humanWB,CoIP

Chem Biol Interact

Therapeutic Targeting of Thioredoxin Reductase 1 Causes Ferroptosis while Potentiating Anti-PD-1 Efficacy in Head and Neck Cancer

Authors - Ming-Shou Hsieh