RRM1-Specific Polyclonal antibody

RRM1-Specific Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 19686-1-AP

Synonyms

RRM1, RR1, RIR1, ribonucleotide reductase M1, Ribonucleoside-diphosphate reductase subunit M1

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Validation Data Gallery

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  WB analysis of A549 using 19686-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 19686-1-AP (RRM1-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19686-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19686-1-AP (RRM1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 19686-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19686-1-AP (RRM1-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 19686-1-AP

  Immunofluorescent analysis of HepG2 cells, using RRM1 antibody 19686-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells, HeLa cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19686-1-AP targets RRM1-Specific in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: ribonucleotide reductase M1
• Calculated Molecular Weight: 90 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: NM_001033
• Gene Symbol: RRM1
• Gene ID (NCBI): 6240
• RRID: AB_10697654
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RRM1, also named as RR1, belongs to the ribonucleoside diphosphate reductase large chain family. RRM1 provides the precursors necessary for DNA synthesis. RRM1 catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.RRM1 is a key enzyme in DNA synthesis: low level expression of RRM1 is associated with poor survival. RRM1 is a prognostic marker of NSCLC. The antibody is specfic to RRM1.