RREB1-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

RREB1-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ChIP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 20280-1-AP

Synonyms

RREB1, RREB 1, Ras-responsive element-binding protein 1, Raf-responsive zinc finger protein LZ321, LZ321

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Validation Data Gallery

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  WB analysis using 20280-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20280-1-AP (RREB1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20280-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20280-1-AP (RREB1-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 20280-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 20280-1-AP (RREB1-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 20280-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20280-1-AP (RREB1-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 20280-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 20280-1-AP (RREB1-Specific antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20280-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20280-1-AP (RREB1-Specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 20280-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20280-1-AP (RREB1-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: BxPC-3 cells, SH-SY5Y cells, HeLa cells
• Positive IHC detected in: human stomach tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• ChIP: See 1 publications below

Product Information

20280-1-AP targets RREB1-Specific in WB, IHC, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: ras responsive element binding protein 1
• Calculated Molecular Weight: 181 kDa
• Observed Molecular Weight: 160-181 kDa, 250 kDa
• GenBank Accession Number: NM_001003698
• Gene Symbol: RREB1
• Gene ID (NCBI): 6239
• RRID: AB_2878661
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RREB1, also named as FINB and Zep-1, belongs to the krueppel C2H2-type zinc-finger protein family. RREB1 is a transcription factor that binds specifically to the RAS-responsive elements (RRE) of gene promoters. It may be involved in Ras/Raf-mediated cell differentiation by enhancing calcitonin expression. RREB1 represses the angiotensinogen gene. RREB1 can induce expression of p53 and represse p16 which associated with the expression of tumour. It has been reported that the RREB1 can be a candidate gene for type 2 diabetes associated end-stage kidney disease. To some extent, the decrease of zinc in human pancreatic adenocarcinoma is associated with the downregulation of RREB1. RREB1 has some isoforms with 250 kDa, 180-200 kDa and 140-160 kDa protein.(PMID: 21703425, 15067362, 25050557, 25027322)

Publications

Species	Application	Title
human	WB,IHC	Cell Cycle; Knockdown of RREB1 inhibits cell proliferation via enhanced p16 expression in gastric cancer.; Authors - Qi Gao ; KD Validated
human	ChIP,WB	Cell Rep; Oncogenic KRAS effector USP13 promotes metastasis in non-small cell lung cancer through deubiquitinating β-catenin; Authors - Yanguan Guo ; KD Validated