RRBP1 Recombinant antibody, PBS Only

RRBP1 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230159G12

Cat no : 82890-2-PBS

Synonyms

Ribosome-binding protein 1, ES/130-related protein, ES/130 related protein, 230159G12, 180 kDa ribosome receptor homolog

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Validation Data Gallery

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  IF Staining of HepG2 using 82890-2-RR (same clone as 82890-2-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using RRBP1 antibody (82890-2-RR, Clone: 230159G12 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). This data was developed using the same antibody clone with 82890-2-PBS in a different storage buffer formulation.

• 

  FC experiment of A549 using 82890-2-RR (same clone as 82890-2-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug Anti-Human RRBP1 (82890-2-RR, Clone:230159G12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82890-2-PBS in a different storage buffer formulation.

• 

  FC experiment of U2OS using 82890-2-RR (same clone as 82890-2-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug Anti-Human RRBP1 (82890-2-RR, Clone:230159G12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82890-2-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82890-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82890-2-RR against Human RRBP1 were performed. The affinity constant is 1.29 nM.

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Product Information

82890-2-PBS targets RRBP1 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RRBP1 fusion protein Ag16648
• Full Name: ribosome binding protein 1 homolog 180kDa (dog)
• Calculated Molecular Weight: 1410 aa, 152 kDa
• Observed Molecular Weight: 108 kDa, 152 kDa
• GenBank Accession Number: BC128577
• Gene Symbol: RRBP1
• Gene ID (NCBI): 6238
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Ribosome-binding protein 1 (RRBP1) is an endoplasmic reticulum membrane protein. It is a 1410 amino acid protein composed of a hydrophobic NH2-terminus that includes a transmembrane domain, a highly conserved tandem repeat (ribosome-binding domain), and an acidic coiled-coil COOH-terminal domain. RRBP1 acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane. It is also involved in early cardiac morphogenesis. (PMID: 22709790; 8812507; 7686155)