RRAS Monoclonal antibody, PBS Only

RRAS Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, Rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E1A12

Cat no : 66959-1-PBS

Synonyms

p23, Ras related protein R Ras, RRAS

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Validation Data Gallery

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  WB analysis of A549 using 66959-1-Ig (same clone as 66959-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 66959-1-Ig (RRAS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66959-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 66959-1-Ig (same clone as 66959-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66959-1-Ig (RRAS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66959-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HT-29 using 66959-1-Ig (same clone as 66959-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66959-1-Ig (RRAS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66959-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 66959-1-Ig (same clone as 66959-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66959-1-Ig (RRAS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66959-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 66959-1-Ig (same clone as 66959-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66959-1-Ig (RRAS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66959-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66959-1-PBS targets RRAS in WB, Indirect ELISA applications and shows reactivity with Human, mouse, Rat samples.

• Tested Reactivity: Human, mouse, Rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: RRAS fusion protein Ag26564
• Full Name: related RAS viral (r-ras) oncogene homolog
• Calculated Molecular Weight: 218 aa, 23 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC016318
• Gene Symbol: RRAS
• Gene ID (NCBI): 6237
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

RRAS belongs to the Ras subfamily of small G-proteins, which are frequently implicated in cell growth and differentiation.RRAS is a small GTPase involved in diverse processes including angiogenesis, vascular homeostasis and regeneration, cell adhesion, and neuronal axon guidance. Mutations in this gene are found in many invasive cancers.