RSK3 Polyclonal antibody

• Featured Product
• KD/KO Validated

RSK3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 14446-1-AP

Synonyms

RPS6KA2, MAPKAPK-1c, MAPKAPK 1c, MAPKAP kinase 1c, MAPK-activated protein kinase 1c

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 14446-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14446-1-AP (RSK3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14446-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14446-1-AP (RSK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 14446-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14446-1-AP (RSK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14446-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14446-1-AP (RSK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14446-1-AP

  IP result of anti-RSK3 (IP:14446-1-AP, 4ug; Detection:14446-1-AP 1:800) with HeLa cells lysate 4000ug.

• 

  IHC staining of human lung cancer using 14446-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14446-1-AP (RSK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 14446-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14446-1-AP (RSK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 14446-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14446-1-AP (RSK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 14446-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14446-1-AP (RSK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 14446-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14446-1-AP (RSK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 14446-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14446-1-AP (RSK3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, HeLa cells, MCF-7 cells, MDA-MB-231 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human lung cancer tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• CoIP: See 1 publications below

Product Information

14446-1-AP targets RSK3 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RSK3 fusion protein Ag5862
• Full Name: ribosomal protein S6 kinase, 90kDa, polypeptide 2
• Calculated Molecular Weight: 83 kDa
• Observed Molecular Weight: 83 kDa
• GenBank Accession Number: BC002363
• Gene Symbol: RSK3
• Gene ID (NCBI): 6196
• RRID: AB_2285316
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RSK3, also named as RPS6KA2, belongs to the protein kinase superfamily. RSK3 is a serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of transcription factors, regulates translation, and mediates cellular proliferation, survival, and differentiation. In addition RSK3 may function as tumor suppressor in epithelial ovarian cancer cells(Uniprot). The molecular weight of RSK3 is 83 kDa.

Publications

Species	Application	Title
human	WB	Theranostics; Kartogenin hydrolysis product 4-aminobiphenyl distributes to cartilage and mediates cartilage regeneration.; Authors - Shuai Zhang
mouse	WB,IHC	Theranostics; RSK-3 promotes cartilage regeneration via interacting with rpS6 in cartilage stem/progenitor cells.; Authors - Shuai Zhang ; KD Validated
human	IHC	Epigenomics; Multiomics analysis identifies key genes and pathways related to N6-methyladenosine RNA modification in ovarian cancer.; Authors - Haoya Xu
human	CoIP	Cell Death Discov; MYSM1 induces apoptosis and sensitizes TNBC cells to cisplatin via RSK3-phospho-BAD pathway.; Authors - Xiaolin Guan
human	WB	J Med Chem; Discovery, Optimization, and Structure-Activity Relationship Study of Novel and Potent RSK4 Inhibitors as Promising Agents for the Treatment of Esophageal Squamous Cell Carcinoma; Authors - Yuan Yuan