S6 Ribosomal protein Polyclonal antibody

S6 Ribosomal protein Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, IP, RIP, ELISA

Conjugate

Unconjugated

Publications

28

Reviews (1)

Cat no : 14823-1-AP

Synonyms

40S ribosomal protein S6, Cell and organelle markers, Phosphoprotein NP33, ribosomal protein S6, Ribosome Marker, RPS6, S6 ribosomal protein

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Validation Data Gallery

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  WB analysis of K-562 using 14823-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14823-1-AP (S6 Ribosomal protein antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14823-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14823-1-AP (S6 Ribosomal protein antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 14823-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14823-1-AP (S6 Ribosomal protein antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 14823-1-AP

  IP result of anti-S6 Ribosomal protein (IP:14823-1-AP, 4ug; Detection:14823-1-AP 1:400) with MCF-7 cells lysate 1040ug.

• 

  IHC staining of human lung cancer using 14823-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14823-1-AP (S6 Ribosomal protein antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 14823-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14823-1-AP (S6 Ribosomal protein antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 14823-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14823-1-AP (S6 Ribosomal protein antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 14823-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14823-1-AP (S6 Ribosomal protein antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: K-562 cells, MCF-7 cells, mouse kidney tissue
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human lung cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 25 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Product Information

14823-1-AP targets S6 Ribosomal protein in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: S6 Ribosomal protein fusion protein Ag6599
• Full Name: ribosomal protein S6
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 29-32 kDa
• GenBank Accession Number: BC000524
• Gene Symbol: RPS6
• Gene ID (NCBI): 6194
• RRID: AB_2181025
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.

Publications

Species	Application	Title
human	WB	Cell Res; Nuclear UHRF1 is a gate-keeper of cellular AMPK activity and function.; Authors - Xiang Xu
human	WB	Nat Commun; Pharmacological inhibition of Lin28 promotes ketogenesis and restores lipid homeostasis in models of non-alcoholic fatty liver disease; Authors - Evangelia Lekka
human	WB	Nat Commun; The short isoform of the host antiviral protein ZAP acts as an inhibitor of SARS-CoV-2 programmed ribosomal frameshifting.; Authors - Matthias M Zimmer
human	WB	Mol Ther; RNA m6A methylation regulates the dissemination of cancer cells via modulating expression and membrane localization of β-catenin.; Authors - Jiexin Li
mouse	WB	Development; MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis; Authors -
human	WB	Oncotarget; Effective use of PI3K inhibitor BKM120 and PARP inhibitor Olaparib to treat PIK3CA mutant ovarian cancer.; Authors - Dong Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wei (Verified Customer) (01-30-2020)

Strong, clear band as predicted around 30 kDa.

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart