S6 Ribosomal protein Polyclonal antibody

S6 Ribosomal protein Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14823-1-AP

Synonyms

40S ribosomal protein S6, Cell and organelle markers, Phosphoprotein NP33, ribosomal protein S6, Ribosome Marker, RPS6, S6 ribosomal protein



Tested Applications

Positive WB detected inK-562 cells, MCF-7 cells, mouse kidney tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14823-1-AP targets S6 Ribosomal protein in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen S6 Ribosomal protein fusion protein Ag6599
Full Name ribosomal protein S6
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 29-32 kDa
GenBank Accession NumberBC000524
Gene Symbol RPS6
Gene ID (NCBI) 6194
RRIDAB_2181025
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.

Protocols

Product Specific Protocols
WB protocol for S6 Ribosomal protein antibody 14823-1-APDownload protocol
IHC protocol for S6 Ribosomal protein antibody 14823-1-APDownload protocol
IP protocol for S6 Ribosomal protein antibody 14823-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Res

Nuclear UHRF1 is a gate-keeper of cellular AMPK activity and function.

Authors - Xiang Xu
humanWB

Nat Commun

Pharmacological inhibition of Lin28 promotes ketogenesis and restores lipid homeostasis in models of non-alcoholic fatty liver disease

Authors - Evangelia Lekka
humanWB

Nat Commun

The short isoform of the host antiviral protein ZAP acts as an inhibitor of SARS-CoV-2 programmed ribosomal frameshifting.

Authors - Matthias M Zimmer
humanWB

Mol Ther

RNA m6A methylation regulates the dissemination of cancer cells via modulating expression and membrane localization of β-catenin.

Authors - Jiexin Li
mouseWB

Development

MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis

Authors -
humanWB

Oncotarget

Effective use of PI3K inhibitor BKM120 and PARP inhibitor Olaparib to treat PIK3CA mutant ovarian cancer.

Authors - Dong Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Wei (Verified Customer) (01-30-2020)

Strong, clear band as predicted around 30 kDa.

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart