RPS4Y1 Polyclonal antibody

RPS4Y1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17296-1-AP

Synonyms

Small ribosomal subunit protein eS4, Y isoform 1, RPS4Y, PRO2646, 40S ribosomal protein S4

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Validation Data Gallery

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  WB analysis of DU 145 using 17296-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 17296-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 17296-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17296-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 17296-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 17296-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 17296-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 17296-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human appendicitis using 17296-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17296-1-AP (RPS4Y1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 17296-1-AP

  Immunofluorescent analysis of HepG2 cells, using RPS4Y1 antibody 17296-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: DU 145 cells, A2780 cells, HeLa cells, mouse ovary tissue, mouse testis tissue, SKOV-3 cells
• Positive IHC detected in: human appendicitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

17296-1-AP targets RPS4Y1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPS4Y1 fusion protein Ag11176
• Full Name: ribosomal protein S4, Y-linked 1
• Calculated Molecular Weight: 263 aa, 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC010286
• Gene Symbol: RPS4Y1
• Gene ID (NCBI): 6192
• RRID: AB_2180889
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPS4Y1, a Y-linked gene in the human, encodes a subunit of ribosomal protein S4, a component of the 40S subunit and 60S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene, RPS4Y2 and the ribosomal protein S4, X-linked (RPS4X). RPS4Y maps to a 90-kb segment that has been implicated in Turner syndrome. As a homolog with RPS4X, the antibody can recognize endogenous level of RPS4Y and RPS4X, at 29-32kDa.

Publications

Species	Application	Title
human	IF	iScience; Secretion of WNT7A by UC-MSCs assist in promoting the endometrial epithelial regeneration; Authors - Fangbo Liu