RPS3 Polyclonal antibody

RPS3 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC

Conjugate

Unconjugated

Cat no : 15198-1-AP

Synonyms

40S ribosomal protein S3, ribosomal protein S3, Ribosome Marker, RPS3



Tested Applications

Positive WB detected inmouse brain tissue, NIH/3T3 cells, mouse colon tissue, COLO 320 cells, HeLa cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman ovary tumor tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

15198-1-AP targets RPS3 in WB, IP, IHC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityyeast
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPS3 fusion protein Ag7346
Full Name ribosomal protein S3
Calculated molecular weight 27 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC003137
Gene symbol RPS3
Gene ID (NCBI) 6188
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The ribosomal protein S3 is a subunit of 40S ribosomal protein, and has two distinct function. RPS3 acts as a ribosome protein that contributes to the domain of the ribosome where translation is initiated, and involves in repair of UV damage as an endonuclease. Besides binding RNA within the 40S ribosomal subunit, RPS3 participates in transcription, and apoptosis.

Protocols

Product Specific Protocols
WB protocol for RPS3 antibody 15198-1-APDownload protocol
IHC protocol for RPS3 antibody 15198-1-APDownload protocol
IP protocol for RPS3 antibody 15198-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
yeastWB

PLoS Genet

Role of Mex67-Mtr2 in the nuclear export of 40S pre-ribosomes.

Authors - Faza Marius B MB