RPS3 Polyclonal antibody

RPS3 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15198-1-AP

Synonyms

ribosomal protein S3, OK/SW-cl.26, FLJ27450, FLJ26283, EC:4.2.99.18

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Validation Data Gallery

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  WB analysis of mouse brain using 15198-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15198-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 15198-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 15198-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15198-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 15198-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 15198-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 15198-1-AP

  IP result of anti-RPS3 (IP:15198-1-AP, 4ug; Detection:15198-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human colon cancer using 15198-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15198-1-AP (RPS3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15198-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15198-1-AP (RPS3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15198-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15198-1-AP (RPS3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 15198-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15198-1-AP (RPS3 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, NIH/3T3 cells, mouse colon tissue, COLO 320 cells, HeLa cells, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

15198-1-AP targets RPS3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: yeast
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPS3 fusion protein Ag7346
• Full Name: ribosomal protein S3
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC003137
• Gene Symbol: RPS3
• Gene ID (NCBI): 6188
• RRID: AB_2253905
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ribosomal protein S3 is a subunit of 40S ribosomal protein, and has two distinct function. RPS3 acts as a ribosome protein that contributes to the domain of the ribosome where translation is initiated, and involves in repair of UV damage as an endonuclease. Besides binding RNA within the 40S ribosomal subunit, RPS3 participates in transcription, and apoptosis.

Publications

Species	Application	Title
yeast	WB	PLoS Genet; Role of Mex67-Mtr2 in the nuclear export of 40S pre-ribosomes.; Authors - Faza Marius B MB