mouse brain tissue were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 15198-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 15198-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
COLO 320 cells were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 15198-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse colon using 15198-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
mouse colon tissue were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 15198-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
rat brain tissue were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15198-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 15198-1-AP
IP result of anti-RPS3 (IP:15198-1-AP, 4ug; Detection:15198-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
IP result of anti-RPS3 (IP:15198-1-AP, 4ug; Detection:15198-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
IHC staining of human colon cancer using 15198-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15198-1-AP (RPS3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15198-1-AP (RPS3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 15198-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15198-1-AP (RPS3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15198-1-AP (RPS3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 15198-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15198-1-AP (RPS3 Antibody) at dilution of 1:50 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15198-1-AP (RPS3 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon cancer using 15198-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15198-1-AP (RPS3 Antibody) at dilution of 1:50 (under 40x lens).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15198-1-AP (RPS3 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of MCF-7 using 15198-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RPS3 antibody (15198-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RPS3 antibody (15198-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
mouse brain tissue, NIH/3T3 cells, mouse colon tissue, COLO 320 cells, HeLa cells, rat brain tissue
Positive IP detected in
mouse brain tissue
Positive IHC detected in
human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
MCF-7 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:2000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:200-1:800
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
The ribosomal protein S3 is a subunit of 40S ribosomal protein, and has two distinct function. RPS3 acts as a ribosome protein that contributes to the domain of the ribosome where translation is initiated, and involves in repair of UV damage as an endonuclease. Besides binding RNA within the 40S ribosomal subunit, RPS3 participates in transcription, and apoptosis.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 15198-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 15198-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 15198-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse colon using 15198-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 15198-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15198-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15198-1-AP (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human colon cancer using 15198-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15198-1-AP (RPS3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 15198-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15198-1-AP (RPS3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 15198-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15198-1-AP (RPS3 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon cancer using 15198-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15198-1-AP (RPS3 Antibody) at dilution of 1:50 (under 40x lens).
IP Figures
IP experiment of mouse brain using 15198-1-AP
IP result of anti-RPS3 (IP:15198-1-AP, 4ug; Detection:15198-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
IF/ICC Figures
IF Staining of MCF-7 using 15198-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RPS3 antibody (15198-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
RPS3 Polyclonal antibody
Catalog Number
15198-1-AP
Citations
1
Dilutions
WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:200-1:800 IF/ICC : 1:200-1:800
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat, yeast
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
