RPS3 Polyclonal antibody, PBS Only

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  WB analysis using 11990-1-AP (same clone as 11990-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11990-1-AP (RPS3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11990-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 11990-1-AP (same clone as 11990-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11990-1-AP (RPS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11990-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-12 using 11990-1-AP (same clone as 11990-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 11990-1-AP (RPS3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11990-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 11990-1-AP (same clone as 11990-1-PBS)

  IP result of anti-RPS3 (IP:11990-1-AP, 3ug; Detection:11990-1-AP 1:600) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 11990-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 11990-1-AP (same clone as 11990-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 11990-1-AP (RPS3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11990-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 11990-1-AP (same clone as 11990-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 11990-1-AP (RPS3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11990-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 11990-1-AP (same clone as 11990-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RPS3 antibody (11990-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11990-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

RPS3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 11990-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

ribosomal protein S3, OK/SW-cl.26, FLJ27450, FLJ26283, EC:4.2.99.18

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Product Information

11990-1-PBS targets RPS3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3918

  Product name: Recombinant human RPS3 protein

  Source: e coli.-derived, Unknow

  Tag: N/A

  Domain: c16032 of BC034149

  Sequence:

• Full Name: ribosomal protein S3
• Calculated Molecular Weight: 26 aa, 7 kDa
• Observed Molecular Weight: 26.7 kDa
• GenBank Accession Number: BC034149
• Gene Symbol: RPS3
• Gene ID (NCBI): 6188
• RRID: AB_2180758
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P23396
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

40S ribosomal protein S3 (RPS3), also named as SW-cl.26, is a 243 amino acid protein,which contains one KH type-2 domain and belongs to the ribosomal protein S3P family. RPS3 localizes in the cytoplasm. RPS3 is identified in a IGF2BP1-dependent mRNP granule complex, which contains untranslated mRNAs. RPS3 plays a role in repairing various DNA damage acting as a repair UV endonuclease. Nuclear accumulation of RPS3 results in an increase in DNA repair activity to some extent, thereby sustaining neuronal survival.