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RPS3 Polyclonal antibody

RPS3 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11990-1-AP

Synonyms

40S ribosomal protein S3, ribosomal protein S3, Ribosome Marker, RPS3



Tested Applications

Positive WB detected inMCF-7 cells, HEK-293 cells, mouse testis tissue, Neuro-2a cells, NIH/3T3 cells, PC-12 cells
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11990-1-AP targets RPS3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPS3 fusion protein Ag3918
Full Name ribosomal protein S3
Calculated molecular weight 26 aa, 7 kDa
Observed molecular weight 26.7 kDa
GenBank accession numberBC034149
Gene symbol RPS3
Gene ID (NCBI) 6188
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

40S ribosomal protein S3 (RPS3), also named as SW-cl.26, is a 243 amino acid protein,which contains one KH type-2 domain and belongs to the ribosomal protein S3P family. RPS3 localizes in the cytoplasm. RPS3 is identified in a IGF2BP1-dependent mRNP granule complex, which contains untranslated mRNAs. RPS3 plays a role in repairing various DNA damage acting as a repair UV endonuclease. Nuclear accumulation of RPS3 results in an increase in DNA repair activity to some extent, thereby sustaining neuronal survival.

Protocols

Product Specific Protocols
WB protocol for RPS3 antibody 11990-1-APDownload protocol
IHC protocol for RPS3 antibody 11990-1-APDownload protocol
IP protocol for RPS3 antibody 11990-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Hepat Med

Mice with null mutation of Ceacam I develop nonalcoholic steatohepatitis.

Authors - Ghosh Sumona S
humanWB

Pathogens

Hsp90 Interacts with the Bacterial Effector NleH1.

Authors - Miaomiao Wu
mouseWB

Cells

Translation Efficiency and Degradation of ER-Associated mRNAs Modulated by ER-Anchored poly(A)-Specific Ribonuclease (PARN).

Authors - Tian-Li Duan
humanIP

Cell Rep

The N-terminal domain of SARS-CoV-2 nsp1 plays key roles in suppression of cellular gene expression and preservation of viral gene expression.

Authors - Aaron S Mendez
pigIF

J Anim Sci Biotechnol

Glyphosate exposure deteriorates oocyte meiotic maturation via induction of organelle dysfunctions in pigs.

Authors - Chunhua Xing
humanWB

Pathogens

SseL Deubiquitinates RPS3 to Inhibit Its Nuclear Translocation.

Authors - Miaomiao Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Boyan (Verified Customer) (12-16-2021)

This antibody recognized a clean band at 27 kd.

  • Applications: Western Blot
FH

Huanzhou (Verified Customer) (11-26-2018)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Cl-16