SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15871-1-AP (RPS27L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15871-1-AP (RPS27L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 15871-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 15871-1-AP (RPS27L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
HepG2 cells were subjected to SDS PAGE followed by western blot with 15871-1-AP (RPS27L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 15871-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 15871-1-AP (RPS27L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
human heart tissue were subjected to SDS PAGE followed by western blot with 15871-1-AP (RPS27L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 15871-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15871-1-AP (RPS27L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15871-1-AP (RPS27L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of SH-SY5Y using 15871-1-AP
IP result of anti-RPS27L (IP:15871-1-AP, 4ug; Detection:15871-1-AP 1:300) with SH-SY5Y cells lysate 2160 ug.
IP result of anti-RPS27L (IP:15871-1-AP, 4ug; Detection:15871-1-AP 1:300) with SH-SY5Y cells lysate 2160 ug.
IHC staining of human stomach using 15871-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 15871-1-AP (RPS27L antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 15871-1-AP (RPS27L antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of SH-SY5Y using 15871-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed SH-SY5Y cells using 15871-1-AP (RPS27L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (10% Formaldehyde) fixed SH-SY5Y cells using 15871-1-AP (RPS27L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
SH-SY5Y cells, mouse brain tissue, human heart tissue, HepG2 cells
Positive IP detected in
SH-SY5Y cells
Positive IHC detected in
human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
SH-SY5Y cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:200-1:1000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15871-1-AP (RPS27L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 15871-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 15871-1-AP (RPS27L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 15871-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 15871-1-AP (RPS27L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 15871-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15871-1-AP (RPS27L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human stomach using 15871-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 15871-1-AP (RPS27L antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of SH-SY5Y using 15871-1-AP
IP result of anti-RPS27L (IP:15871-1-AP, 4ug; Detection:15871-1-AP 1:300) with SH-SY5Y cells lysate 2160 ug.
IF/ICC Figures
IF Staining of SH-SY5Y using 15871-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed SH-SY5Y cells using 15871-1-AP (RPS27L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs.
We have selected the top cited antibodies from these vendors for you to compare.
Proteintech
RPS27L Polyclonal antibody
Catalog Number
15871-1-AP
Citations
2
Dilutions
WB : 1:200-1:1000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:50-1:500
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
