RPS27L Polyclonal antibody

RPS27L Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15871-1-AP

Synonyms

Small ribosomal subunit protein eS27-like, RPS, Ribosomal protein eS27-like, 40S ribosomal protein S27-like

---

Validation Data Gallery

• 

  WB analysis of SH-SY5Y using 15871-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15871-1-AP (RPS27L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15871-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15871-1-AP (RPS27L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 15871-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15871-1-AP (RPS27L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15871-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15871-1-AP (RPS27L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SH-SY5Y using 15871-1-AP

  IP result of anti-RPS27L (IP:15871-1-AP, 4ug; Detection:15871-1-AP 1:300) with SH-SY5Y cells lysate 2160 ug.

• 

  IHC staining of human stomach using 15871-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 15871-1-AP (RPS27L antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 15871-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed SH-SY5Y cells using 15871-1-AP (RPS27L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: SH-SY5Y cells, mouse brain tissue, human heart tissue, HepG2 cells
• Positive IP detected in: SH-SY5Y cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

15871-1-AP targets RPS27L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPS27L fusion protein Ag8628
• Full Name: ribosomal protein S27-like
• Calculated Molecular Weight: 84 aa, 10 kDa
• Observed Molecular Weight: 10 kDa
• GenBank Accession Number: BC003667
• Gene Symbol: RPS27L
• Gene ID (NCBI): 51065
• RRID: AB_2253903
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Elife; Subfunctionalized expression drives evolutionary retention of ribosomal protein paralogs Rps27 and Rps27l in vertebrates; Authors - Adele Francis Xu
human	WB	J Mol Biol; Defective Human SRP Induces Protein Quality Control and Triggers Stress Response; Authors - Elena B Tikhonova