RPS18 Recombinant antibody, PBS Only

RPS18 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240004C10

Cat no : 83327-1-PBS

Synonyms

Ke-3, KE3, KE 3, HKE3, D6S218E

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Validation Data Gallery

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  IF Staining of HepG2 using 83327-1-RR (same clone as 83327-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPS18 antibody (83327-1-RR, Clone: 240004C10 ) at dilution of 1:0 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83327-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 83327-1-RR (same clone as 83327-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RPS18 Recombinant antibody (83327-1-RR, Clone:240004C10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83327-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83327-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83327-1-RR against Human RPS18 were performed. The affinity constant is 0.47 nM.

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Product Information

The immunogen of 83327-1-PBS is RPS18 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RPS18 fusion protein Ag12505
• Full Name: ribosomal protein S18
• Calculated Molecular Weight: 152 aa, 18 kDa
• GenBank Accession Number: BC106063
• Gene Symbol: RPS18
• Gene ID (NCBI): 6222
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.