RPS15 Polyclonal antibody

RPS15 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

6

Reviews (1)

Cat no : 14957-1-AP

Synonyms

Small ribosomal subunit protein uS19, RIG protein, RIG, ribosomal protein S15, 40S ribosomal protein S15

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Validation Data Gallery

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  WB analysis of human liver using 14957-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14957-1-AP (RPS15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14957-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14957-1-AP (RPS15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 14957-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14957-1-AP (RPS15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14957-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14957-1-AP (RPS15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 14957-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 14957-1-AP (RPS15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 14957-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14957-1-AP (RPS15 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 14957-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14957-1-AP (RPS15 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human colon cancer using 14957-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14957-1-AP (RPS15 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 14957-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14957-1-AP (RPS15 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 14957-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14957-1-AP (RPS15 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 14957-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14957-1-AP (RPS15 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 14957-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 14957-1-AP (RPS15 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 14957-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14957-1-AP (RPS15 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 14957-1-AP

  Immunofluorescent analysis of Hela cells, using RPS15 antibody 14957-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: human liver tissue, HeLa cells, L02 cells, mouse brain tissue, mouse liver tissue
• Positive IHC detected in: human lymphoma tissue, mouse liver tissue, rat liver tissue, human liver cancer tissue, human colon cancer tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 1 publications below

Product Information

14957-1-AP targets RPS15 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPS15 fusion protein Ag6795
• Full Name: ribosomal protein S15
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC064908
• Gene Symbol: RPS15
• Gene ID (NCBI): 6209
• RRID: AB_2180163
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPS15, also named as RIG protein or Small ribosomal subunit protein uS19, is a 145 amino acid protein, which belongs to the universal ribosomal protein uS19 family. RPS15 is a ribosomal small subunit assembly and involved in nuclear-transcribed mRNA catabolic process, nonsense-mediated decay.

Publications

Species	Application	Title
human	WB	Curr Biol; RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.; Authors - Long Jiang
human	IF	Cell Prolif; An artificial intelligence network-guided signature for predicting outcome and immunotherapy response in lung adenocarcinoma patients based on 26 machine learning algorithms; Authors - Nan Zhang
human	WB	Elife; The molecular basis of coupling between poly(A)-tail length and translational efficiency.; Authors - Kehui Xiang
human		Cell Rep; FKBP10 Regulates Protein Translation to Sustain Lung Cancer Growth.; Authors - Giorgio Ramadori
human	WB	Elife; The unfolded protein response and endoplasmic reticulum protein targeting machineries converge on the stress sensor IRE1.; Authors - Diego Acosta-Alvear
human	WB	iScience; TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment; Authors - Magdalena Wolczyk

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Advait (Verified Customer) (02-22-2019)

4% PFA fixationBlocking buffer: BSA with 0.05% SaponinIncubate at 4 degrees overnight for best staining quality.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: HeLa

RPS15 Antibody Western Blot,Immunofluorescence, validation (1:100 dilution) in HeLa (Cat no:14957-1-AP)