RPS14 Polyclonal antibody

RPS14 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 16683-1-AP

Synonyms

Small ribosomal subunit protein uS11, RPS 14, ribosomal protein S14, PRO2640, EMTB

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Validation Data Gallery

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  WB analysis using 16683-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16683-1-AP (RPS14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16683-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16683-1-AP (RPS14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 16683-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16683-1-AP (RPS14 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16683-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16683-1-AP (RPS14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 16683-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16683-1-AP (RPS14 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 16683-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16683-1-AP (RPS14 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 16683-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 16683-1-AP (RPS14 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS cells using 16683-1-AP

  IF result of 16683-1-AP (anti-RPS14) in U2OS cell by Dr. Nancy Kedersha. (cytosolic small ribosomal and nucleolus; Green).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse liver tissue, HepG2 cells, rat liver tissue
• Positive IHC detected in: human liver tissue, human colon; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Product Information

16683-1-AP targets RPS14 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, zebrafish, arabidopsis
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPS14 fusion protein Ag10012
• Full Name: ribosomal protein S14
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC006784
• Gene Symbol: RPS14
• Gene ID (NCBI): 6208
• RRID: AB_2180158
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The mammalian ribosome consists of 4 RNA species and approximately 80 different proteins. The ribosomal proteins are encoded by complex gene families that include at least 1 active intron-containing gene and multiple processed pseudogenes[ PMID 3785212]. The RPS14 gene, encoding a ribosomal protein that belongs to the 40S ribosome, and mapping to the commonly deleted region (CDR) of the 5q- syndrome, shows haploinsufficiency in the CD34+ cells of patients with this disorder[PMID:22430631].

Publications

Species	Application	Title
human	IF	Nucleic Acids Res; Selenite targets eIF4E-binding protein-1 to inhibit translation initiation and induce the assembly of non-canonical stress granules.; Authors - Fujimura Ken K
human	WB	Acta Neuropathol Commun; Altered ribosomal function and protein synthesis caused by tau.; Authors - Harrison Tudor Evans
Arabidopsis	WB	J Exp Bot; The in vivo functions of ARPF2 and ARRS1 in ribosomal RNA processing and ribosome biogenesis in Arabidopsis.; Authors - Ilyeong Choi
human	WB	Free Radic Biol Med; Identification of interacting partners of Human Mpv17-like protein with a mitigating effect of mitochondrial dysfunction through mtDNA damage.; Authors - Reiko Iida
	WB	J Exp Bot; Retention of OsNMD3 in the cytoplasm disturbs protein synthesis efficiency and affects plant development in rice.; Authors - Yanyun Shi
zebrafish	IF	Blood Adv; TLR7 ligation augments hematopoiesis in Rps14 (uS11) deficiency via paradoxical suppression of inflammatory signaling.; Authors - Oscar A Peña