RPS12 Polyclonal antibody

• Featured Product
• KD/KO Validated

RPS12 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 16490-1-AP

Synonyms

Small ribosomal subunit protein eS12, ribosomal protein S12, 40S ribosomal protein S12

---

Validation Data Gallery

• 

  WB analysis of NIH/3T3 using 16490-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16490-1-AP (RPS12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16490-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16490-1-AP (RPS12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 16490-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16490-1-AP (RPS12 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16490-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16490-1-AP (RPS12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16490-1-AP

  IP result of anti-RPS12 (IP:16490-1-AP, 4ug; Detection:16490-1-AP 1:1000) with HeLa cells lysate 2920 ug.

• 

  IHC staining of human colon cancer using 16490-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 16490-1-AP (RPS12 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 16490-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 16490-1-AP (RPS12 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 16490-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using RPS12 antibody (16490-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: NIH/3T3 cells, HeLa cells, MCF-7 cells, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

16490-1-AP targets RPS12 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, drosophila
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPS12 fusion protein Ag9615
• Full Name: ribosomal protein S12
• Calculated Molecular Weight: 132 aa, 15 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC017321
• Gene Symbol: RPS12
• Gene ID (NCBI): 6206
• RRID: AB_2146233
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPS12 (ribosomal protein S12) is a plastid ribosomal protein which is a part of the 30S ribosomal subunit. Together with S4 and S5 plays an important role in translational accuracy.This antibody specifically recognizes the 15kd RPS12 protein.

Publications

Species	Application	Title
mouse	WB	Mol Cell; RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages; Authors - Teodorus Theo Susanto
	WB	Dev Cell; Axonal endoplasmic reticulum tubules control local translation via P180/RRBP1-mediated ribosome interactions; Authors - Max Koppers
		Dev Cell; Progressive Differentiation and Instructive Capacities of Amniotic Fluid and Cerebrospinal Fluid Proteomes following Neural Tube Closure.; Authors - Kevin F Chau
mouse	IHC	Elife; Downregulation of ribosome biogenesis during early forebrain development.; Authors - Kevin F Chau
mouse	IHC	Development; Recombineering-based dissection of flanking and paralogous Hox gene functions in mouse reproductive tracts.; Authors - Raines Anna M AM
drosophila	IF	Sci Rep; HumanaFly: high-throughput transgenesis and expression of breast cancer transcripts in Drosophila eye discovers the RPS12-Wingless signaling axis.; Authors - Vladimir L Katanaev