RPS11 Polyclonal antibody

RPS11 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15942-1-AP

Synonyms

Small ribosomal subunit protein uS17, ribosomal protein S11, 40S ribosomal protein S11

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Validation Data Gallery

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  WB analysis using 15942-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15942-1-AP (RPS11 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 15942-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15942-1-AP (RPS11 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, MDA-MB-453s cells, ROS1728 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15942-1-AP targets RPS11 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPS11 fusion protein Ag8346
• Full Name: ribosomal protein S11
• Calculated Molecular Weight: 158 aa, 18 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC007945
• Gene Symbol: RPS11
• Gene ID (NCBI): 6205
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPS11 is a ribosomal protein involved in ribosome biogenesis. It is a component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.