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  • KD/KO Validated

RPLP1 Polyclonal antibody

RPLP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21636-1-AP

Synonyms

P1, ribosomal protein, large, P1, RPLP1, RPP1, RRP1



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21636-1-AP targets RPLP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPLP1 fusion protein Ag16268
Full Name ribosomal protein, large, P1
Calculated Molecular Weight 12 kDa
Observed Molecular Weight 15-20 kDa
GenBank Accession NumberBC003369
Gene Symbol RPLP1
Gene ID (NCBI) 6176
RRIDAB_2878894
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

60S acidic ribosomal protein P1 (RRP1) is a 114 amino acid protein, which belongs to the ribosomal protein L12P family. RRP1 plays an important role in the elongation step of protein synthesis. The molecular weight of modified RRP1 is 15-20 kDa.

Protocols

Product Specific Protocols
WB protocol for RPLP1 antibody 21636-1-APDownload protocol
IHC protocol for RPLP1 antibody 21636-1-APDownload protocol
IF protocol for RPLP1 antibody 21636-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

Virulence

RPLP1, an NS4B-interacting protein, enhances production of CSFV through promoting translation of viral genome.

Authors - Longxiang Zhang
  • KD Validated
mouseWB

Mol Hum Reprod

60S acidic ribosomal protein P1 (RPLP1) is elevated in human endometriotic tissue and in a murine model of endometriosis and is essential for endometriotic epithelial cell survival in vitro.

Authors - Zahraa Alali
humanWB

Int J Mol Sci

RPLP1 Is Up-Regulated in Human Adenomyosis and Endometrial Adenocarcinoma Epithelial Cells and Is Essential for Cell Survival and Migration In Vitro

Authors - Riley Peterson
humanWB,IHC

Am J Cancer Res

Unveiling the prognostic implications of RPLP1 upregulation in osteosarcoma

Authors - Xinhui Du
humanWB,IF

Nat Commun

RPLP1 restricts HIV-1 transcription by disrupting C/EBPβ binding to the LTR

Authors - Weijing Yang