RPLP0 Polyclonal antibody

RPLP0 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugate

Unconjugated

Publications

23

Reviews (1)

Cat no : 11290-2-AP

Synonyms

ribosomal protein, large, P0, PRLP0, P0, Large ribosomal subunit protein uL10, L10E

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Validation Data Gallery

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  WB analysis using 11290-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11290-2-AP (RPLP0 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11290-2-AP

  IP result of anti-RPLP0 (IP:11290-2-AP, 4ug; Detection:11290-2-AP 1:500) with HeLa cells lysate 2680 ug.

• 

  IP experiment of HeLa using 11290-2-AP

  IP result of anti-RPLP0 (IP:11290-2-AP, 4ug; Detection:11290-2-AP 1:500) with HeLa cells lysate 3200ug.

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  IHC staining of human colon cancer using 11290-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11290-2-AP (RPLP0 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HeLa using 11290-2-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11290-2-AP (RPLP0 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: C6 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 16 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• ELISA: See 3 publications below
• RIP: See 1 publications below

Product Information

11290-2-AP targets RPLP0 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPLP0 fusion protein Ag1829
• Full Name: ribosomal protein, large, P0
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC009867
• Gene Symbol: RPLP0
• Gene ID (NCBI): 6175
• RRID: AB_2254064
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPLP0 is first identified as a cytosolic protein which takes a part in the ribosomal stalk and interacts with RPLP1 and RPLP2. It also has potential roles in molecular transport from outside into the cells. It generally presents in multiple copies on the ribosome and have isoelectric points in the range of pH 3 to 5, in contrast to most ribosomal proteins, which are single copy and basic

Publications

Species	Application	Title
human	WB	Cell Metab; miR-378(∗) mediates metabolic shift in breast cancer cells via the PGC-1β/ERRγ transcriptional pathway.; Authors - Lillian J Eichner
human	WB	Genes Dev; The homeobox protein Prox1 is a negative modulator of ERR{alpha}/PGC-1{alpha} bioenergetic functions.; Authors - Charest-Marcotte Alexis A
human	WB	J Cell Biol; Nucleolar protein B23/nucleophosmin regulates the vertebrate SUMO pathway through SENP3 and SENP5 proteases.; Authors - Yun Chawon C
mouse	WB	EMBO J; Decreased synthesis of ribosomal proteins in tauopathy revealed by non-canonical amino acid labelling.; Authors - Harrison Tudor Evans
human	IHC	Proc Natl Acad Sci U S A; Local translation in nuclear condensate amyloid bodies.; Authors - Phaedra R Theodoridis
human	WB	PLoS Genet; Genomic convergence among ERRα, PROX1, and BMAL1 in the control of metabolic clock outputs.; Authors - Dufour Catherine R CR

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Trinh (Verified Customer) (06-02-2020)

good antibody

• Applications: Western Blot
• Cell Tissue Type: HUVEC