RPL8 Polyclonal antibody

• Featured Product
• KD/KO Validated

RPL8 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 16981-1-AP

Synonyms

60S ribosomal protein L8, ribosomal protein L8, RPL8

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Validation Data Gallery

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  WB analysis using 16981-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16981-1-AP (RPL8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16981-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16981-1-AP (RPL8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HepG2 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Product Information

16981-1-AP targets RPL8 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPL8 fusion protein Ag9957
• Full Name: ribosomal protein L8
• Calculated Molecular Weight: 257 aa, 28 kDa
• Observed Molecular Weight: 28-34 kDa
• GenBank Accession Number: BC012197
• Gene Symbol: RPL8
• Gene ID (NCBI): 6132
• RRID: AB_2182071
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Mol Cell; RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages; Authors - Teodorus Theo Susanto
human	IF	Cell Discov; Ribosomal RNA regulates chromosome clustering during mitosis.; Authors - Kai Ma
human	WB	Genes (Basel); Transcriptional Regulation of CD40 Expression by 4 Ribosomal Proteins via a Functional SNP on a Disease-Associated CD40 Locus.; Authors - Meijuan Zou ; KD Validated