RPL39 Polyclonal antibody

• Featured Product
• KD/KO Validated

RPL39 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 14990-1-AP

Synonyms

ribosomal protein L39, Large ribosomal subunit protein eL39, eL39, 60S ribosomal protein L39

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Validation Data Gallery

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  WB analysis of HepG2 using 14990-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14990-1-AP (RPL39 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 14990-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14990-1-AP (RPL39 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 14990-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14990-1-AP (RPL39 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver cancer using 14990-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14990-1-AP (RPL39 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 14990-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL39 antibody (14990-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, human liver tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Product Information

14990-1-AP targets RPL39 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPL39 fusion protein Ag6975
• Full Name: ribosomal protein L39
• Calculated Molecular Weight: 6 kDa
• Observed Molecular Weight: 6 kDa
• GenBank Accession Number: BC001019
• Gene Symbol: RPL39
• Gene ID (NCBI): 6170
• RRID: AB_2238670
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Cell; Early Scanning of Nascent Polypeptides inside the Ribosomal Tunnel by NAC.; Authors - Martin Gamerdinger
human	FC	Transl Res; Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication.; Authors - Mahmoud Dabbah
zebrafish	IF	Elife; In vivo proximity labeling identifies cardiomyocyte protein networks during zebrafish heart regeneration.; Authors - Mira I Pronobis
human	IF	Cell Discov; Ribosomal RNA regulates chromosome clustering during mitosis.; Authors - Kai Ma
human	WB,IHC,IF	Front Immunol; Identification and validation of a 17-gene signature to improve the survival prediction of gliomas; Authors - Shiao Tong ; KD Validated
mouse	WB,IP,IF	iScience; Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis.; Authors - Qianxing Zou ; KO Validated