RPL32 Polyclonal antibody

RPL32 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 16629-1-AP

Synonyms

60S ribosomal protein L32, PP9932, ribosomal protein L32, RPL32

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Validation Data Gallery

• 

  WB analysis of HeLa using 16629-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16629-1-AP (RPL32 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 16629-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 16629-1-AP (RPL32 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16629-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16629-1-AP (RPL32 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 16629-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16629-1-AP (RPL32 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16629-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16629-1-AP (RPL32 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, mouse lung tissue, HepG2 cells, A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16629-1-AP targets RPL32 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPL32 fusion protein Ag9960
• Full Name: ribosomal protein L32
• Calculated Molecular Weight: 135 aa, 16 kDa
• Observed Molecular Weight: 16-18 kDa
• GenBank Accession Number: BC070209
• Gene Symbol: RPL32
• Gene ID (NCBI): 6161
• RRID: AB_2254034
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.