RPL3 Polyclonal antibody

• Featured Product
• KD/KO Validated

RPL3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

11

Reviews (1)

Cat no : 11005-1-AP

Synonyms

TARBP B, ribosomal protein L3, OK/SW-cl.32, Large ribosomal subunit protein uL3, HIV-1 TAR RNA-binding protein B

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Validation Data Gallery

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  WB analysis of HeLa using 11005-1-AP

  WB result of RPL3 antibody (11005-1-AP, 1:2000) with si-control and si-RPL3 transfected HeLa cells..

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  WB analysis using 11005-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11005-1-AP (RPL3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 11005-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11005-1-AP (RPL3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 11005-1-AP

  IP result of anti-RPL3 (IP:11005-1-AP, 4ug; Detection:11005-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human prostate cancer using 11005-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11005-1-AP (RPL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 11005-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11005-1-AP (RPL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11005-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL3 antibody (11005-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: Jurkat cells, human kidney tissue, HeLa cells, Raji cells, human placenta tissue, mouse kidney tissue
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

11005-1-AP targets RPL3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPL3 fusion protein Ag1436
• Full Name: ribosomal protein L3
• Calculated Molecular Weight: 46/27 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC012786
• Gene Symbol: RPL3
• Gene ID (NCBI): 6122
• RRID: AB_2181760
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL3, also named as TARBP-B and MGC104284, belongs to the ribosomal protein L3P family. This protein is a component of the 60S subunit. It can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. This is a rabbit polyclonal antibody raised against the C terminus of human RPL3.

Publications

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A; Preventing translational inhibition from ribosomal protein insufficiency by a herpes simplex virus-encoded ribosome-associated protein.; Authors - Elizabeth I Vink ; KD Validated
human	WB	Elife; METTL18-mediated histidine methylation of RPL3 modulates translation elongation for proteostasis maintenance.; Authors - Eriko Matsuura-Suzuki
human	WB	Cell Discov; Ribosomal RNA regulates chromosome clustering during mitosis.; Authors - Kai Ma
mouse	WB	Aging (Albany NY); RNA-seq analysis of the key long noncoding RNAs and mRNAs related to cognitive impairment after cardiac arrest and cardiopulmonary resuscitation.; Authors - Chan Chen
human	WB	Nucleic Acid Ther; Knockdown of Muscle-Specific Ribosomal Protein L3-Like Enhances Muscle Function in Healthy and Dystrophic Mice.; Authors - Betty R Kao
human	IHC	Carcinogenesis; DUOX2 promotes the progression of colorectal cancer cells by regulating the AKT pathway and interacting with RPL3.; Authors - Xue Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Abe (Verified Customer) (04-25-2024)

Worked well for IF at 1:500 overnight at 4c in RPE-1 cells.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: RPE-1