RPL29 Recombinant antibody

RPL29 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse , rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240062F9

Cat no : 83377-1-RR

Synonyms

60S ribosomal protein L29, HIP, HUMRPL29, ribosomal protein L29, RPL29

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Validation Data Gallery

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  WB analysis using 83377-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83377-1-RR (RPL29 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 83377-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL29 antibody (83377-1-RR, Clone: 240062F9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HepG2 using 83377-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL29 antibody (83377-1-RR, Clone: 240062F9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HeLa using 83377-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RPL29 Recombinant antibody (83377-1-RR, Clone:240062F9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, PC-3 cells, U-87 MG cells, C6 cells, NIH/3T3 cells,
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83377-1-RR is RPL29 Fusion Protein expressed in E. coli.

• Tested Reactivity: Human, mouse , rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RPL29 fusion protein Ag34100
• Full Name: ribosomal protein L29
• Calculated Molecular Weight: 159 aa, 18 kDa
• Observed Molecular Weight: 20-25 kDa
• GenBank Accession Number: BC008926
• Gene Symbol: RPL29
• Gene ID (NCBI): 6159
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribosomal protein L29 (Rpl29) is a component of the large (60S) ribosomal subunit which is abundantly expressed in all cell types, plays a regulatory role in translation efficiency and is essential for protein synthesis (PMID: 17195189). In contrast to adult tissues, RPL29 displays a much more widespread pattern of expression throughout embryonic development, and high levels of RPL29 are found in all developing organs. Several reports showed that RPL29 expression is up-regulated in human carcinomas and constitutes a candidate marker for abnormal growth when overexpressed(PMID: 9788632, 10383165).