RPL29 Polyclonal antibody, PBS Only

RPL29 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15799-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 15799-1-AP (same clone as 15799-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15799-1-AP (RPL29 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15799-1-PBS in a different storage buffer formulation.

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  WB analysis using 15799-1-AP (same clone as 15799-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15799-1-AP (RPL29 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15799-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 15799-1-AP (same clone as 15799-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15799-1-AP (RPL29 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15799-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 15799-1-AP (same clone as 15799-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15799-1-AP (RPL29 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15799-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 15799-1-AP (same clone as 15799-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15799-1-AP (RPL29 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15799-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 15799-1-AP (same clone as 15799-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15799-1-AP (RPL29 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15799-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 15799-1-AP (same clone as 15799-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15799-1-AP (RPL29 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15799-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 15799-1-AP (same clone as 15799-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15799-1-AP (RPL29 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15799-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 15799-1-AP (same clone as 15799-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RPL29 antibody (15799-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 15799-1-PBS in a different storage buffer formulation.

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Product Information

15799-1-PBS targets RPL29 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPL29 fusion protein Ag8510
• Full Name: ribosomal protein L29
• Calculated Molecular Weight: 159 aa, 18 kDa
• Observed Molecular Weight: 20-25 kDa
• GenBank Accession Number: BC008926
• Gene Symbol: RPL29
• Gene ID (NCBI): 6159
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Ribosomal protein L29 (Rpl29) is a component of the large (60S) ribosomal subunit which is abundantly expressed in all cell types, plays a regulatory role in translation efficiency and is essential for protein synthesis (PMID: 17195189). In contrast to adult tissues, RPL29 displays a much more widespread pattern of expression throughout embryonic development, and high levels of RPL29 are found in all developing organs. Several reports showed that RPL29 expression is up-regulated in human carcinomas and constitutes a candidate marker for abnormal growth when overexpressed(PMID: 9788632, 10383165).The antibody can detect the band around 22kDa.