RPL27A Polyclonal antibody

• Featured Product
• KD/KO Validated

RPL27A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 16002-1-AP

Synonyms

ribosomal protein L27a, Large ribosomal subunit protein uL15, 60S ribosomal protein L27a

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Validation Data Gallery

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  WB analysis of A549 using 16002-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 16002-1-AP (RPL27A Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  WB analysis of A549 using 16002-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 16002-1-AP (RPL27A Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IHC staining of mouse stomach using 16002-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 16002-1-AP (RPL27A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 16002-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 16002-1-AP (RPL27A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 16002-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 16002-1-AP (RPL27A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells
• Positive IHC detected in: mouse stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• FC: See 1 publications below

Product Information

16002-1-AP targets RPL27A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPL27A fusion protein Ag8596
• Full Name: ribosomal protein L27a
• Calculated Molecular Weight: 148 aa, 18 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC005326
• Gene Symbol: RPL27A
• Gene ID (NCBI): 6157
• RRID: AB_2878206
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,FC	Transl Res; Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication.; Authors - Mahmoud Dabbah
human	WB	Bioengineered; tRF-19-W4PU732S promotes breast cancer cell malignant activity by targeting inhibition of RPL27A (ribosomal protein-L27A).; Authors - Zhengxiang Zhang ; KD Validated