RPL27 Polyclonal antibody

RPL27 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 14980-1-AP

Synonyms

60S ribosomal protein L27, ribosomal protein L27, RPL27

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Validation Data Gallery

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  WB analysis of mouse liver using 14980-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14980-1-AP (RPL27 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 14980-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14980-1-AP (RPL27 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 14980-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14980-1-AP (RPL27 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver cancer using 14980-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14980-1-AP (RPL27 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse liver tissue, human liver tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

14980-1-AP targets RPL27 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPL27 fusion protein Ag6893
• Full Name: ribosomal protein L27
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC001700
• Gene Symbol: RPL27
• Gene ID (NCBI): 6155
• RRID: AB_2181742
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPL27, also named as 60S ribosomal protein L27, is a 136 amino acid protein, which contains one KOW domain and belongs to the ribosomal protein L27e family. The function of RPL27 is still non-known. RPL27 may play a role in protein translation. Like most ribosomal proteins, Ribosomal Protein L27 exists as multiple processed pseudogenes that are scattered throughout the genome.

Publications

Species	Application	Title
human	IHC	Proc Natl Acad Sci U S A; Local translation in nuclear condensate amyloid bodies.; Authors - Phaedra R Theodoridis
rat	WB,IHC	J Orthop Translat; Comparative proteomic analysis identifies differentially expressed proteins and reveals potential mechanisms of traumatic heterotopic ossification progression.; Authors - Zhenyuan Wei
rat	WB	Physiol Rep; Aldosterone and angiotensin II induce protein aggregation in renal proximal tubules.; Authors - Muhammad U Cheema
mouse	WB	EMBO Rep; miR-132 suppresses transcription of ribosomal proteins to promote protective Th1 immunity.; Authors - James P Hewitson
rat	WB	Brain Sci; Identification and Validation of Novel Potential Pathogenesis and Biomarkers to Predict the Neurological Outcome after Cardiac Arrest; Authors - Qiang Zhang
mouse	IF	Nat Struct Mol Biol; Nucleolus assembly impairment leads to two-cell transcriptional repression via NPM1-mediated PRC2 recruitment; Authors - Dingfeng Li