RPL23A Polyclonal antibody, PBS Only

RPL23A Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16386-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 16386-1-AP (same clone as 16386-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16386-1-AP (RPL23A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16386-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 16386-1-AP (same clone as 16386-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16386-1-AP (RPL23A antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16386-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human liver using 16386-1-AP (same clone as 16386-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 16386-1-AP (RPL23A antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16386-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 16386-1-AP (same clone as 16386-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 16386-1-AP (RPL23A antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16386-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 16386-1-AP (same clone as 16386-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16386-1-AP (RPL23A antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16386-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human liver using 16386-1-AP (same clone as 16386-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 16386-1-AP (RPL23A antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16386-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 16386-1-AP (same clone as 16386-1-PBS)

  IP result of anti-RPL23A (IP:16386-1-AP, 4ug; Detection:16386-1-AP 1:500) with HepG2 cells lysate 3200ug. This data was developed using the same antibody clone with 16386-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 16386-1-AP (same clone as 16386-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 16386-1-AP (RPL23A antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16386-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 16386-1-AP (same clone as 16386-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 16386-1-AP (RPL23A antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16386-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 16386-1-AP (same clone as 16386-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 16386-1-AP (RPL23A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16386-1-PBS in a different storage buffer formulation.

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Product Information

16386-1-PBS targets RPL23A in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPL23A fusion protein Ag9424
• Full Name: ribosomal protein L23a
• Calculated Molecular Weight: 156 aa, 18 kDa
• Observed Molecular Weight: 18-23 kDa
• GenBank Accession Number: BC014459
• Gene Symbol: RPL23A
• Gene ID (NCBI): 6147
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.