RPL19 Polyclonal antibody

RPL19 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 14701-1-AP

Synonyms

ribosomal protein L19, Large ribosomal subunit protein eL19, 60S ribosomal protein L19

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Validation Data Gallery

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  WB analysis of HeLa using 14701-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14701-1-AP (RPL19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 14701-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14701-1-AP (RPL19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14701-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14701-1-AP (RPL19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 14701-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14701-1-AP (RPL19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 14701-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14701-1-AP (RPL19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14701-1-AP

  IP result of anti-RPL19 (IP:14701-1-AP, 4ug; Detection:14701-1-AP 1:3000) with HeLa cells lysate 1560 ug.

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  IHC staining of human brain using 14701-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14701-1-AP (RPL19 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 14701-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14701-1-AP (RPL19 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse brain using 14701-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14701-1-AP (RPL19 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14701-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14701-1-AP (RPL19 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14701-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 14701-1-AP (RPL19 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 14701-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human RPL19 (14701-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, mouse spleen tissue, mouse testis tissue, PC-3 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human brain tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• FC: See 1 publications below

Product Information

14701-1-AP targets RPL19 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPL19 fusion protein Ag6371
• Full Name: ribosomal protein L19
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC066315
• Gene Symbol: RPL19
• Gene ID (NCBI): 6143
• RRID: AB_2181587
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPL19, also named as 60S ribosomal protein L19, is a 196 amino acid protein, which belongs to the ribosomal protein L19e family. RPL19 encodes a ribosomal protein that is a component of the 60S subunit.

Publications

Species	Application	Title
human	WB,FC	Transl Res; Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication.; Authors - Mahmoud Dabbah
mouse	WB	Dev Cell; Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility.; Authors - Yuqing Lei
mouse	WB	EMBO J; Decreased synthesis of ribosomal proteins in tauopathy revealed by non-canonical amino acid labelling.; Authors - Harrison Tudor Evans
human	WB	Curr Biol; RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.; Authors - Long Jiang
human	WB	Hum Mutat; Frameshift mutation in p53 regulator RPL26 is associated with multiple physical abnormalities and a specific pre-ribosomal RNA processing defect in diamond-blackfan anemia.; Authors - Gazda Hanna T HT
		EMBO J; Decreased synthesis of ribosomal proteins in tauopathy revealed by non-canonical amino acid labelling.; Authors - Harrison Tudor Evans