RPL19 Polyclonal antibody

RPL19 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 14701-1-AP

Synonyms

ribosomal protein L19, Large ribosomal subunit protein eL19, 60S ribosomal protein L19



Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, mouse spleen tissue, mouse testis tissue, PC-3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14701-1-AP targets RPL19 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL19 fusion protein Ag6371
Full Name ribosomal protein L19
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC066315
Gene Symbol RPL19
Gene ID (NCBI) 6143
RRIDAB_2181587
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPL19, also named as 60S ribosomal protein L19, is a 196 amino acid protein, which belongs to the ribosomal protein L19e family. RPL19 encodes a ribosomal protein that is a component of the 60S subunit.

Protocols

Product Specific Protocols
WB protocol for RPL19 antibody 14701-1-APDownload protocol
IHC protocol for RPL19 antibody 14701-1-APDownload protocol
IF protocol for RPL19 antibody 14701-1-APDownload protocol
IP protocol for RPL19 antibody 14701-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,FC

Transl Res

Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication.

Authors - Mahmoud Dabbah
mouseWB

Dev Cell

Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility.

Authors - Yuqing Lei
mouseWB

EMBO J

Decreased synthesis of ribosomal proteins in tauopathy revealed by non-canonical amino acid labelling.

Authors - Harrison Tudor Evans
humanWB

Curr Biol

RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.

Authors - Long Jiang
humanWB

Hum Mutat

Frameshift mutation in p53 regulator RPL26 is associated with multiple physical abnormalities and a specific pre-ribosomal RNA processing defect in diamond-blackfan anemia.

Authors - Gazda Hanna T HT

EMBO J

Decreased synthesis of ribosomal proteins in tauopathy revealed by non-canonical amino acid labelling.

Authors - Harrison Tudor Evans