RPL18A Recombinant antibody

RPL18A Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240841B1

Cat no : 83928-1-RR

Synonyms

ribosomal protein L18a, Large ribosomal subunit protein eL20, 60S ribosomal protein L18a, 240841B1

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Validation Data Gallery

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  WB analysis using 83928-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83928-1-RR (RPL18A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83928-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83928-1-RR against Human RPL18A were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, PC-3 cells, MCF-7 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83928-1-RR is RPL18A Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RPL18A fusion protein Ag6251
• Full Name: ribosomal protein L18a
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC066319
• Gene Symbol: RPL18A
• Gene ID (NCBI): 6142
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPL18A, also named as 60S ribosomal protein L18a(Ribosomal Protein L18a), located on 19p13.11, is a protein coding gene. RPL18A is a member of the L18AE family of ribosomal proteins that is a component of the 60S subunit. The encoded protein may play a role in viral replication by interacting with the hepatitis C virus internal ribosome entry site (IRES). Diseases such as Hepatitis C Virus and Omsk Hemorrhagic Fever are associated with RPL18A. The molecular weight of RPL18A is 23 kDa.