RPL18A Polyclonal antibody, PBS Only

RPL18A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14653-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HepG2 using 14653-1-AP (same clone as 14653-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14653-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 14653-1-AP (same clone as 14653-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14653-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 14653-1-AP (same clone as 14653-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14653-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 14653-1-AP (same clone as 14653-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14653-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 14653-1-AP (same clone as 14653-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14653-1-AP (RPL18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14653-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 14653-1-AP (same clone as 14653-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14653-1-AP (RPL18A antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14653-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 14653-1-AP (same clone as 14653-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14653-1-AP (RPL18A antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14653-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 14653-1-AP (same clone as 14653-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL18A antibody (14653-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14653-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 14653-1-AP (same clone as 14653-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using RPL18A antibody 14653-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14653-1-PBS in a different storage buffer formulation.

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Product Information

14653-1-PBS targets RPL18A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPL18A fusion protein Ag6251
• Full Name: ribosomal protein L18a
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC066319
• Gene Symbol: RPL18A
• Gene ID (NCBI): 6142
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

RPL18A, also named as 60S ribosomal protein L18a(Ribosomal Protein L18a), located on 19p13.11, is a protein coding gene. RPL18A is a member of the L18AE family of ribosomal proteins that is a component of the 60S subunit. The encoded protein may play a role in viral replication by interacting with the hepatitis C virus internal ribosome entry site (IRES). Diseases such as Hepatitis C Virus and Omsk Hemorrhagic Fever are associated with RPL18A. The molecular weight of RPL18A is 23 kDa.