RPL15 Recombinant antibody

RPL15 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242029E3

Cat no : 84900-1-RR

Synonyms

RPL10, ribosomal protein L15, Large ribosomal subunit protein eL15, EC45, 60S ribosomal protein L15

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Validation Data Gallery

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  WB analysis using 84900-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84900-1-RR (RPL15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, K-562 cells, COLO 320 cells, NCI-H1299 cells, mouse colon tissue, rat colon tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84900-1-RR targets RPL15 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RPL15 fusion protein Ag9948
• Full Name: ribosomal protein L15
• Calculated Molecular Weight: 204 aa, 24 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC070328
• Gene Symbol: RPL15
• Gene ID (NCBI): 6138
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPL15 (ribosomal protein L15) is a component of the ribosomal large subunit 60S, participating in the assembly process of ribosomal subunits and involved in the processing of rRNA. Moreover, RPL15 deletion will lead to abnormalities in the biogenesis of ribosomal subunits. The abnormal expression or mutation of RPL15 protein is also closely related to the occurrence and development of many diseases. RPL15 was highly expressed in gastric cancer cell lines, and interference with the expression of RPL15 could inhibit gastric cancer cell growth. In addition, RPL15 in skin squamous cell carcinoma tissues and pancreatic cancer cell lines decreased sharply, which had a great relationship with the survival rate. PMID:35410346