RPL12 Monoclonal antibody

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  WB analysis using 68110-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68110-1-Ig (RPL12 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  IHC staining of rat brain using 68110-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68110-1-Ig (RPL12 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat stomach using 68110-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 68110-1-Ig (RPL12 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 68110-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68110-1-Ig (RPL12 antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 68110-1-Ig

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 68110-1-Ig (RPL12 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 68110-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68110-1-Ig (RPL12 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 68110-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 68110-1-Ig (RPL12 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RPL12 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 68110-1-Ig

Clone No.2C9B3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ribosomal protein L12, Large ribosomal subunit protein uL11, 60S ribosomal protein L12, 2C9B3

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Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
• Positive IHC detected in: mouse brain tissue, human endometrial cancer tissue, human placenta tissue, mouse stomach tissue, rat brain tissue, rat stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochemistry (IHC): IHC : 1:8000-1:32000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68110-1-Ig targets RPL12 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RPL12 fusion protein Ag6580
• Full Name: ribosomal protein L12
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC050644
• Gene Symbol: RPL12
• Gene ID (NCBI): 6136
• RRID: AB_2923639
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P30050
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.