RPL11 Recombinant antibody

RPL11 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242147D7

Cat no : 84670-3-RR

Synonyms

GIG34, DBA7, CLL-associated antigen KW-12, CLL associated antigen KW 12, 60S ribosomal protein L11

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Validation Data Gallery

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  WB analysis using 84670-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84670-3-RR (RPL11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84670-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84670-3-RR against Human RPL11 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells, BxPC-3 cells, mouse pancreas tissue, mouse liver tissue, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84670-3-RR targets RPL11 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RPL11 fusion protein Ag9356
• Full Name: ribosomal protein L11
• Calculated Molecular Weight: 177 aa, 20 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC018970
• Gene Symbol: RPL11
• Gene ID (NCBI): 6135
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribosomal protein L11 is one of 80 mammalian ribosome proteins. It can bind to 5S ribosomal RNA, for it's required for rRNA maturation and formation of the 60S ribosomal subunit. It can inhibit 40S or 60S ribosome biogenesis via mediated p53 induction. Once retained by PICT1 in the nucleolus, RPL11 involve in regulation of the MDM2-P53 pathway and promotion of tumor progression.