RPL10 Polyclonal antibody

RPL10 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 17013-1-AP

Synonyms

60S ribosomal protein L10, DXS648, DXS648E, Laminin receptor homolog, NOV, Protein QM, QM, ribosomal protein L10, RPL10, Tumor suppressor QM

---

Validation Data Gallery

• 

  WB analysis using 17013-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17013-1-AP (RPL10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17013-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17013-1-AP (RPL10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 17013-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17013-1-AP (RPL10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17013-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17013-1-AP (RPL10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 17013-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17013-1-AP (RPL10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 17013-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17013-1-AP (RPL10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: HeLa cells, COLO 320 cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

17013-1-AP targets RPL10 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPL10 fusion protein Ag9969
• Full Name: ribosomal protein L10
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC003358
• Gene Symbol: RPL10
• Gene ID (NCBI): 6134
• RRID: AB_2181278
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPL10, also named as Large ribosomal subunit protein uL16, is a 214 amino acid protein, which belongs to the universal ribosomal protein uL16 family. RPL10 is a component of the large ribosomal subunit and is down-regulated during adipocyte, kidney, and heart differentiation. RPL10 shares 98% amino acid sequence with RPL10L, so RPL10 antibody is able to recognize RPL10 and RPL10L protein.

Publications

Species	Application	Title
human	WB	Cell Discov; Ribosomal RNA regulates chromosome clustering during mitosis.; Authors - Kai Ma
human	WB,IHC	Transl Oncol; Identification of metastasis-associated exoDEPs in colorectal cancer using label-free proteomics.; Authors - Xinlu Liu
mouse	IF	Development; Lin28-mediated promotion of protein synthesis is critical for neural progenitor cell maintenance and brain development in mice.; Authors - Stephanie Herrlinger
human	WB	Brain; GGC repeat expansion in NOTCH2NLC induces dysfunction in ribosome biogenesis and translation; Authors - Yu Fan
human	WB	Cardiovasc Res; The translational landscape of human vascular smooth muscle cells identifies novel short ORF-encoded peptide regulators for phenotype alteration; Authors - Kang Li