RPIA Polyclonal antibody

RPIA Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13010-1-AP

Synonyms

RPI, Ribose-5-phosphate isomerase, ribose 5 phosphate isomerase A, Phosphoriboisomerase, EC:5.3.1.6

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Validation Data Gallery

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  WB analysis using 13010-1-AP

  mouse spleen tissues were subjected to SDS PAGE followed by western blot with 13010-1-AP (RPIA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 13010-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13010-1-AP (RPIA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 13010-1-AP

  Immunofluorescent analysis of HepG2 cells, using RPIA antibody 13010-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse spleen tissue, HEK-293 cells, MCF-7 cells, PC-3 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

13010-1-AP targets RPIA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPIA fusion protein Ag3699
• Full Name: ribose 5-phosphate isomerase A
• Calculated Molecular Weight: 311 aa, 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC015529
• Gene Symbol: RPIA
• Gene ID (NCBI): 22934
• RRID: AB_10638301
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPIA(Ribose-5-phosphate isomerase) is also named as RPI and belongs to the ribose 5-phosphate isomerase family. It is involved in pentose-phosphate shunt, non-oxidative branch. It is an enzyme in the pentose-phosphate pathway, appears to be determined by a single structural locus and is probably a dimer(PMID:7396409).

Publications

Species	Application	Title
human,mouse	WB,IHC	Int J Biol Sci; Diallyl disulfide induces DNA damage and growth inhibition in colorectal cancer cells by promoting POU2F1 ubiquitination; Authors - Longzheng Xia