RPH3AL Polyclonal antibody, PBS Only

RPH3AL Polyclonal Antibody for WB, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15297-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of human liver using 15297-1-AP (same clone as 15297-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 15297-1-AP (RPH3AL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15297-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 15297-1-AP (same clone as 15297-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 15297-1-AP (RPH3AL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15297-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 15297-1-AP (same clone as 15297-1-PBS)

  IP result of anti-RPH3AL (IP:15297-1-AP, 4ug; Detection:15297-1-AP 1:500) with HepG2 cells lysate 2400ug. This data was developed using the same antibody clone with 15297-1-PBS in a different storage buffer formulation.

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Product Information

15297-1-PBS targets RPH3AL in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPH3AL fusion protein Ag7552
• Full Name: rabphilin 3A-like (without C2 domains)
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC005153
• Gene Symbol: RPH3AL
• Gene ID (NCBI): 9501
• RRID: AB_2181270
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Rabphilin-3A-like (RPH3AL), also known as Noc2, is a Rab effector protein with a molecular weight of 38 kDa. It is expressed abundantly in endocrine cells but at low levels in exocrine tissues, playing a direct regulatory role in calcium-ion-dependent exocytosis in both endocrine and exocrine cells, and also having a key role in INS secretion by pancreatic cells. RPH3AL functions as a potential RAB3B effector protein in epithelial cells, and RPH3AL gene is likely a tumor suppressor.