RPE65 Recombinant antibody

RPE65 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240518F1

Cat no : 83861-1-RR

Synonyms

Lutein isomerase, LCA2, EC:5.3.3.22, EC:3.1.1.64, All-trans-retinyl-palmitate hydrolase

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Validation Data Gallery

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  WB analysis using 83861-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83861-1-RR (RPE65 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, PC-3 cells, Y79 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83861-1-RR is RPE65 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RPE65 fusion protein Ag12356
• Full Name: retinal pigment epithelium-specific protein 65kDa
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 65 kDa, 61 kDa
• GenBank Accession Number: BC075036
• Gene Symbol: RPE65
• Gene ID (NCBI): 6121
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPE65(Retinal pigment epithelium-specific 65 kDa protein) is also named as retinoid isomerohydrolase with the calculated molecular weight og 61 kDa and belongs to the carotenoid oxygenase family. It is a microsomal protein with isomerase activity in the retinoid visual cycle that playing a role in the isomerisation of all-trans (vitamin A) to 11-cis retinal, the chromophore of the visual pigments. It also might be involved in retinoid uptake of keratinocytes.