RPE Polyclonal antibody

• Featured Product
• KD/KO Validated

RPE Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12168-2-AP

Synonyms

Ribulose-phosphate 3-epimerase, Ribulose-5-phosphate-3-epimerase, HUSSY-17, EC:5.1.3.1

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Validation Data Gallery

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  WB analysis of HEK-293T using 12168-2-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 12168-2-AP (RPE antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 12168-2-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12168-2-AP (RPE antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12168-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12168-2-AP (RPE antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12168-2-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12168-2-AP (RPE antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of RAW 264.7 using 12168-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using RPE antibody (12168-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293T cells, mouse thymus tissue, HeLa cells, K-562 cells
• Positive IF/ICC detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

12168-2-AP targets RPE in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPE fusion protein Ag2810
• Full Name: ribulose-5-phosphate-3-epimerase
• Calculated Molecular Weight: 228 aa, 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC016764
• Gene Symbol: RPE
• Gene ID (NCBI): 6120
• RRID: AB_2253939
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Rep; mTOR Overcomes Multiple Metabolic Restrictions to Enable HIV-1 Reverse Transcription and Intracellular Transport.; Authors - Harry E Taylor
human	WB	Nat Metab; Salvage of ribose from uridine or RNA supports glycolysis in nutrient-limited conditions; Authors - Owen S Skinner ; KO Validated